Abcam, ab265574, Human SLC20A2 (PIT2) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC20A2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC20A2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SLC20A2 also known as PIT2 is a sodium-dependent phosphate transporter protein with a molecular mass of approximately 80 kDa. It facilitates the uptake of inorganic phosphate into cells which is critical for various cellular functions. This protein is expressed in many tissues including the brain kidneys and lungs. In the brain SLC20A2 plays an important role in maintaining phosphate homeostasis which is necessary for neuronal activity and development.
Biological function summary
The function of this transporter protein is to maintain phosphate balance at the cellular level. It does not form any known complex with other proteins but works by itself to regulate phosphate uptake. SLC20A2 activity is essential for normal bone mineralization and energy metabolism. The phosphate transported by SLC20A2 is used in ATP production and biological signaling processes highlighting its importance in cellular metabolism.
Pathways
The role of SLC20A2 is particularly evident in the phosphate metabolism pathway. It works alongside other family proteins like SLC20A1 to regulate phosphate levels. Alteration in these pathways can impact cellular processes such as glycolysis and oxidative phosphorylation emphasizing the need for precise phosphate balance. SLC20A2 interacts with various signaling pathways that control cellular growth and differentiation showing its significance in energy and phosphate-related pathways.
Abnormal SLC20A2 function has been linked to idiopathic basal ganglia calcification a disorder characterized by calcium deposit in brain regions affecting motor control. Mutations in the SLC20A2 gene can disrupt phosphate transport leading to abnormal calcium accumulation. This disorder often involves other proteins such as XPR1 which can also be implicated in phosphate transport. Understanding the role of SLC20A2 in this context helps in developing targeted therapies for better disease management.