Abcam, ab265684, Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PPP1R2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPP1R2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Protein Phosphatase 1 Inhibitor Subunit 2 often abbreviated as PPP1R2 functions as a regulatory protein that inhibits the activity of Protein Phosphatase 1 (PP1). PP1 is an important enzyme in various cellular processes including cell division and glycogen metabolism. PPP1R2 has a molecular mass of approximately 20 kDa. This inhibitory protein is ubiquitously expressed throughout different tissues indicating its role in fundamental cellular functions.
Biological function summary
PPP1R2 plays a critical role in cellular regulation by modulating PP1 activity. It forms a complex with PP1 in which PPP1R2 binds directly to the catalytic subunit of PP1 inhibiting its phosphatase activity. By controlling PP1 activity PPP1R2 impacts processes like muscle contraction protein synthesis and neuronal signaling making it an active participant in maintaining cellular homeostasis.
Pathways
PPP1R2 functions within important signaling and dephosphorylation pathways. It is notably involved in the glycogen metabolism pathway where it regulates PP1 activity influencing glycogen synthesis and breakdown. Additionally it participates in the cell cycle regulation pathway interacting with other proteins such as PP1 to control cell proliferation and division. The regulation of these pathways by PPP1R2 highlights its importance in key cellular activities.
PPP1R2's dysregulation can associate with several conditions. For instance alterations in PPP1R2 levels have implications in neurodegenerative diseases like Alzheimer's Disease since it affects neuronal protein dephosphorylation. Furthermore abnormal PPP1R2 expression can link to metabolic disorders including Type 2 Diabetes due to its role in glycogen metabolism. In these diseases its interaction with other proteins like PP1 and insulin signaling components is significant highlighting its potential as a therapeutic target.