Abcam, ab265693, Human RAB9A (Rab9) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RAB9A KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RAB9A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rab9 also known as Ras-related protein Rab-9A is a small GTPase and part of the Rab family with a mass of around 23 kDa. This protein mainly resides in the trans-Golgi network and late endosomes. Rab9 plays an important role in the transport of mannose 6-phosphate receptors between late endosomes and the Golgi apparatus facilitating cargo sorting and vesicular trafficking in cells.
Biological function summary
Rab9 coordinates several intracellular trafficking processes aiding in the regulation of membrane identity. It associates with other effectors such as tail-interacting protein (TIP47) to form complexes on the endosomal surface. These complexes help in vesicular transport ensuring efficient recycling of receptors. Rab9 impacts endosome maturation and transport making it an essential endosome marker.
Pathways
Rab9 influences and is involved in the regulation of the endocytic and secretory pathways. It interacts with proteins like Rab7 another GTPase to manage late endosome and lysosome transport. These interactions underline Rab9's functionalities in intracellular trafficking and recycling processes relying on cooperative interactions with other Rabs and their effectors.
Rab9 plays a role in conditions like Niemann-Pick disease type C and some neurological disorders. Disruption in Rab9 function may affect lipid trafficking and lead to accumulation of cholesterol in late endosomes. This links Rab9 indirectly to proteins such as NPC1 which is known to be implicated in cholesterol homeostasis. Understanding Rab9's involvement can help inform therapeutic strategies targeting these pathways in related diseases.