Abcam, ab265701, Human SH2D4A knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SH2D4A KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 5 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 5 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SH2D4A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SH2D4A also known as Src homology 2 domain-containing protein 4A is a protein involved in signal transduction pathways. It has a molecular mass of approximately 58 kDa. This protein contains an SH2 domain which plays a role in mediating protein-protein interactions by binding to phosphotyrosine-containing motifs. SH2D4A is expressed in various tissues with higher levels observed in immune-related tissues including the spleen and lymph nodes. This expression pattern suggests a functional role in cellular communication and response mechanisms.
Biological function summary
The SH2D4A protein participates in the regulation of immune responses. It is not considered part of a large complex but interacts with other signaling molecules to influence downstream signaling cascades. By engaging with phosphorylated partners SH2D4A modulates pathways important for cell growth proliferation and immune activation. This function positions SH2D4A as an important mediator in cellular signaling networks underpinning immune system function.
Pathways
Research identifies SH2D4A as influencing both the MAPK and JAK-STAT pathways. It interacts with key proteins within these pathways such as MAPK1 which is integral for transmitting extracellular signals into cellular responses. These pathways regulate processes essential for cell division growth and development. SH2D4A's interaction with signaling components supports its role in controlling cellular responses to external stimuli and maintaining cellular homeostasis.
SH2D4A has been linked to conditions impacting the immune system. Abnormalities in this protein's function have correlations with autoimmune diseases and certain types of lymphomas. SH2D4A is connected to proteins like STAT5 which is important in cytokine signaling and immune cell differentiation. Dysregulation of SH2D4A leading to altered interaction with signaling molecules may drive pathological changes seen in these immune-related disorders.