Abcam, ab265724, Human PTPN13 (FAP-1) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PTPN13 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 14. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 14,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PTPN13, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PTPN13 also known as FAP-1 or Fas-associated phosphatase-1 is a non-receptor type protein tyrosine phosphatase with a reported molecular weight of approximately 270 kDa. This enzyme contains several PDZ domains contributing to its interactions with other proteins. PTPN13 is expressed in various tissues including the brain liver and heart indicating its wide-ranging roles in cellular functions.
Biological function summary
PTPN13 plays a critical role in the regulation of apoptosis and cell-cell adhesion. It interacts with components of the cytoskeleton and other signaling proteins functioning as part of protein complexes involved in maintaining cellular structure and signaling. By dephosphorylating specific phosphotyrosine residues PTPN13 controls signaling pathways that determine cell survival and communication.
Pathways
PTPN13 is involved in pathways such as the Fas-mediated apoptosis signaling and insulin signaling pathways. It interacts with proteins like the Fas receptor and IRS1 within these pathways modulating signals that control apoptosis and metabolic responses. PTPN13's activity can affect downstream signaling events influencing cellular outcomes like survival or death in response to external stimuli.
Researchers have linked PTPN13 with cancer and insulin resistance. The protein's interaction with Fas-associated proteins suggests a role in tumorigenesis particularly related to apoptosis evasion. In the context of metabolic disorders PTPN13's function in the insulin signaling pathway connects it to insulin resistance where its regulation of IRS1 affects insulin response. These associations highlight the potential of targeting PTPN13 in therapeutic strategies for these diseases.