Abcam, ab265823, Human SLC38A9 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC38A9 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 5 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 5 bp deletion in exon 3,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC38A9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SLC38A9 also known as SNAT9 functions as an amino acid transporter and sensor within lysosomes. It specifically mediates the transport of essential amino acids and related signals from the lysosomal lumen to the cytoplasm. The protein has a mass of approximately 61 kilodaltons and is primarily expressed in tissues with high metabolic activity like the liver kidneys and brain. This expression pattern supports its role in cellular and metabolic homeostasis.
Biological function summary
This protein serves as a component of the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway. SLC38A9 directly interacts with the lysosomal surface to connect amino acid availability to mTORC1 activation. By doing so it influences cell growth and autophagy processes in response to amino acid levels. As a part of the mTORC1 complex SLC38A9 plays an important function in translating extracellular cues to intracellular responses.
Pathways
SLC38A9 is integral in the amino acid sensing mechanism that regulates the mTORC1 pathway. This pathway controls vital processes like protein synthesis and cell proliferation. The protein partners with other key players in the mTOR signaling pathway including Rag GTPases and Raptor to relay amino acid abundance to the mTORC1 complex. This relationship ensures that cellular growth processes are synchronized with available nutrition resources.
SLC38A9 is implicated in metabolic diseases and cancer. Dysregulation of amino acid sensing and mTORC1 activity mediated through SLC38A9 contributes to metabolic disorders such as obesity and type 2 diabetes. Moreover its involvement with mTORC1 links it to cancer progression as mTORC1 is often upregulated in tumors. This relationship suggests a potential therapeutic interest in targeting SLC38A9 together with proteins like mTOR for disease intervention.