Abcam, ab265828, Human LAMTOR2 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
LAMTOR2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 7 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 7 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LAMTOR2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAMTOR2 also called p14 acts as an important component of the Ragulator complex. With a molecular mass of approximately 16 kDa LAMTOR2 localizes mainly on the late endosomal and lysosomal membranes. This protein aids in the recruitment of other proteins to these membranes facilitating various cellular processes. The expression of LAMTOR2 finds prominence in many tissues especially those with high metabolic activity.
Biological function summary
LAMTOR2 serves in the regulation of mTORC1 signaling which plays a major role in cell growth and metabolism. As a member of the Ragulator complex LAMTOR2 works in concert with other proteins like LAMTOR1 and LAMTOR3 to anchor the Rag GTPases to the lysosomal surface. This provides a platform for the activation of mTORC1 in response to amino acid availability therefore guiding cellular energy and protein synthesis pathways.
Pathways
LAMTOR2 functions integrally in the amino acid-sensing pathway and impacts the mTOR signaling pathway. The Ragulator complex involving LAMTOR2 interacts with Rag GTPases to activate mTORC1 upon amino acid availability bridging nutrient sensing and cellular metabolism control. In doing so it interacts closely with proteins like RagA/RagB coordinating signals that are critical for growth-related cellular functions.
LAMTOR2 disruptions have links to immunodeficiency and some metabolic conditions. Deficient Ragulator function including LAMTOR2 leads to improper mTOR signaling impacting immune cell function and systemic metabolism. Notably this situation associates with diseases like Charcot-Marie-Tooth disease where LAMTOR2's interaction with LAMTOR3 becomes significant due to affected lysosomal positioning and signaling pathways.