Abcam, ab265843, Human POLDIP3 (p46) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
POLDIP3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 1 and 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 1 and 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-POLDIP3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
P46 also known as heat shock protein 46 (HSP46) is a molecular chaperone within the heat shock protein family. It maintains a mass of approximately 46 kDa and is ubiquitously expressed in various tissues. The protein assists in protein folding and prevents the aggregation of misfolded proteins. It acts in cellular stress responses aiding cells in coping with conditions like heat or oxidative stress. Researchers recognize its important role in maintaining cellular protein homeostasis.
Biological function summary
Heat shock protein 46 is integral in cellular protection during stress conditions. It participates in forming complexes with other heat shock proteins enhancing its function in assisting proteins during synthesis and repair. This protein acts in maintaining the stability and function of other proteins preserving cellular health under stress. Its involvement in cellular processes signifies its importance in both cellular functioning and stress response mechanisms.
Pathways
P46 plays a critical role in the heat shock response and protein quality control pathways. It interacts with proteins such as HSP70 and HSP90 which participate in similar pathways ensuring proper folding and stability of newly synthesized proteins. These pathways protect cells from damage during stress connecting p46 to broader physiological responses. Its presence in these pathways emphasizes its significance in cellular recovery and adaptation processes.
P46 connects to neurodegenerative diseases and cancer. In neurodegenerative diseases such as Alzheimer's its function becomes important due to the accumulation of misfolded proteins sharing pathways with proteins like HSP70 which also counteracts protein misfolding. In cancer the overexpression of p46 has been observed linking it to tumor progression where it may stabilize proteins required for tumor growth and survival. These connections highlight how alterations in p46 levels may contribute to disease development and progression.