Abcam, ab265928, Human LANCL1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
LANCL1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and Insertion of the selection cassette in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LANCL1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LANCL1 also known as Lanthionine synthetase component C-like protein 1 is a protein present in the cell and has a mass of approximately 47 kDa. This protein shows expression in various tissues including the brain and adipose tissue. It belongs to the larger LANCL protein family and shows sequence homology with other family members. LANCL1 works intracellularly binding and modulating the activity of different molecules which could influence various cell processes.
Biological function summary
The LANCL1 protein interacts with several intracellular proteins. It might not act alone but rather as part of a multiprotein complex which suggests a role in complex cellular mechanisms. LANCL1's activity affects cellular maintenance and growth. It helps in the regulation of oxidative stress responses by directly impacting key cellular regulators of redox state.
Pathways
The LANCL1 protein plays a role in the oxidative stress response pathway and energy metabolism pathway. It modulates cellular responses to oxidative stress and interacts with proteins such as NRF2 which is important for the antioxidant response. Furthermore it has a potential connection with pathways linked to energy homeostasis aligning with its expression in adipose tissue where it could interact with metabolic proteins like AMPK.
LANCL1 has associations with neurodegenerative diseases and obesity. Aberrant regulation of LANCL1 protein might lead to increased oxidative stress contributing to conditions such as Alzheimer's disease where it could potentially influence the activity of the protein amyloid-beta. Additionally its role in energy metabolism links it to obesity where it might affect proteins critical in lipid storage and adipose tissue function.