Abcam, ab265953, Human ATP5SL knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
DMAC2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2 and 31 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2 and 31 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-DMAC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP5SL also known as the ATP synthase subunit s-like protein interacts with components of the mitochondrial ATP synthase complex. This protein has a mass of approximately 27 kDa. Researchers often detect its expression in tissues with high metabolic rates such as the heart and skeletal muscles. ATP5SL participates in energy production by modulating the efficiency of ATP synthesis.
Biological function summary
The ATP5SL protein plays a significant role in ATP production acting within the mitochondrial ATP synthase complex. As part of this complex it helps synthesize ATP from ADP and inorganic phosphate. ATP5SL works closely with other subunits in the complex to maintain optimal energy conversion necessary for cellular functions. Its proper function is essential in maintaining cellular energy homeostasis.
Pathways
The role of ATP5SL is integral to oxidative phosphorylation and cellular respiration. It interacts with various proteins including ATP5A1 and ATP5B which are critical to the ATP synthase complex. These interactions ensure efficient electron transport chain function and ATP production key steps in cellular respiration and energy metabolism pathways.
ATP5SL can affect conditions related to mitochondrial dysfunctions like mitochondrial encephalomyopathy and cardiomyopathy. Abnormalities in ATP5SL can lead to impaired energy production. Disorders linked to the malfunctioning of ATP synthase often show associations with proteins like MFN2 which plays a role in mitochondrial fusion. Understanding ATP5SL's involvement in these processes provides insights into potential therapeutic targets for mitochondrial-related diseases.