Abcam, ab265975, Human WDR41 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
WDR41 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp deletion in exon 1 and 5 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp deletion in exon 1 and 5 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WDR41, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WDR41 also known as WD repeat domain 41 is a protein that participates in cellular processes through its distinctive structural motif. The protein has multiple WD40 repeats and shows a molecular mass of approximately 74 kDa. WDR41 is found within the cytoplasm and also shows expression in various tissues across the human body including the brain and kidney. This wide expression implies its involvement in essential cellular activities.
Biological function summary
WDR41 contributes to lysosomal function and acts as a component of the PI3P-binding WIPI3-WDR41 complex. The complex plays an important role in autophagy by promoting the formation of autophagosomes which are important for degrading and recycling cellular components. Additionally WDR41 participates in regulation of cellular homeostasis playing a role in maintaining the balance of proteins and organelles within the cell environment.
Pathways
WDR41 plays a significant role in the autophagy and mTOR signaling pathways. These pathways are key in regulating cell growth proliferation and survival. Autophagy involves several proteins and complexes with mTORC1 being a vital regulator that signals the inhibition or promotion of this catabolic process. WDR41 interacts with other proteins such as LC3 and FYCO1 which help to mediate the autophagic response within cells and provide insights into its integration in these critical pathways.
Improper functioning of WDR41 links to neurodegenerative diseases and metabolic syndromes. Disruption in its expression may contribute to the development of conditions such as Parkinson's disease where misregulation of autophagy leads to the accumulation of damaged organelles and proteins. Additionally WDR41 connections with the mTOR pathway can influence type 2 diabetes progression. Its interactions with proteins like ULK1 and Atg13 emphasize its role in these disease contexts highlighting its potential as a target for therapeutic interventions.