Abcam, ab266049, Human TTC3 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TTC3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TTC3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The TTC3 protein also known as Tetratricopeptide Repeat Domain 3 has a molecular weight of approximately 215 kDa. This protein functions as an E3 ubiquitin-protein ligase which means it plays a role in tagging proteins for degradation via the ubiquitin-proteasome system. TTC3 is widely expressed in the brain especially within neurons and is also found in other tissues such as the heart and kidneys. It contains tetratricopeptide repeats which are known for facilitating protein-protein interactions essential for its role in cellular function and signaling.
Biological function summary
TTC3 interacts in various processes associated with cell development and stability. It is not part of a larger protein complex but operates notably in cell cycle progression and neuronal differentiation. TTC3 regulates the stability of proteins by adding ubiquitin molecules which are small regulatory proteins to target proteins. This activity is important for maintaining the cellular environment especially in tissues where precise control over protein levels is necessary.
Pathways
Many cellular pathways are influenced by TTC3 including the PI3K-AKT signaling pathway which mediates several cellular processes such as growth and survival. TTC3 has connections with proteins such as AKT1 through this pathway which helps regulate cell proliferation and neurogenesis. Additionally TTC3 is involved in the stability regulation of cyclin proteins influencing the cell cycle by interacting with components of the cyclin-dependent kinase (CDK) pathway therefore affecting cell cycle checkpoints and transitions.
Evidence shows that changes in TTC3 expression have links to neurological conditions like Down syndrome and Alzheimer's disease. In these disorders altered TTC3 function affects neuronal growth and function likely through dysregulated ubiquitination processes. Additionally TTC3 interacts with proteins like DYRK1A which is also implicated in neurodegenerative conditions and Down syndrome pathophysiology. Altered TTC3 activity therefore contributes to the molecular mechanisms driving these diseases suggesting its potential as a therapeutic target.