Abcam, ab266093, Human ARPP19 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
ARPP19 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 29 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 29 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ARPP19, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARPP-19 also known as cAMP-regulated phosphoprotein 19 is a protein that plays an important role in cellular processes. The protein has a molecular weight of approximately 19 kDa. Its expression can be found in various tissues with significant levels detected in the brain. ARPP-19 undergoes phosphorylation which is essential for its activity in cellular signaling.
Biological function summary
ARPP-19 influences cell cycle regulation and neuronal signaling. The protein does not appear to be part of a larger protein complex. It interacts with protein phosphatase 2A (PP2A) modulating its activity during mitosis. This regulation helps ensure proper cell division and function. The protein also shows involvement in neurodevelopmental pathways contributing to brain development and function.
Pathways
The involvement of ARPP-19 includes regulation within the cAMP signaling pathway and the mitotic spindle assembly checkpoint. It interacts with proteins like PP2A and Greatwall kinase coordinating the phosphorylation status of key mitotic substrates. ARPP-19 affects the transition from interphase to mitosis impacting the signaling cascade that controls this phase.
ARPP-19 has associations with cancer and neurodegenerative diseases. Aberrant phosphorylation of ARPP-19 and related proteins like PP2A can lead to mitotic errors promoting oncogenesis. In neurodegenerative contexts altered ARPP-19 function may affect neuronal signaling and plasticity contributing to conditions such as Alzheimer's disease. Understanding these interactions helps in exploring therapeutic strategies targeting these specific pathways.