Abcam, ab266213, Human GPC4 (Glypican 4) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
GPC4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 1 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 1 bp insertion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-GPC4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glypican 4 also known as GPC4 is a heparan sulfate proteoglycan a member of the glypican family. This protein is anchored to the cell membrane and plays a role in cellular processes by attaching to the cell surface via a glycosylphosphatidylinositol (GPI) anchor. Its mass is approximately 60-70 kDa. Glypican 4 is expressed in various tissues including the brain pancreas kidney and skeletal muscle. In these tissues Glypican 4 is involved in cell communication and modulation of signaling activities at the cell surface.
Biological function summary
Glypican 4 influences cell growth differentiation and morphogenesis. It interacts with extracellular ligands and growth factors playing a role in developmental processes. Glypican 4 is often part of a complex with other signaling molecules including Wnt and fibroblast growth factors. These interactions help regulate processes essential for organ development and tissue patterning. Glypican 4 through these molecular associations influences signaling pathways that affect both cellular architecture and function.
Pathways
Glypican 4 plays a role in Wnt signaling and Hedgehog pathways. These pathways are critical for embryonic development and cell fate determination. Within the Wnt signaling pathway Glypican 4 interacts with the Wnt protein modulating its distribution and activity on the cell surface. In the Hedgehog pathway Glypican 4 helps modulate hedgehog proteins like Sonic Hedgehog facilitating their movement and interaction with cell surface receptors.
Glypican 4 is connected to some cancers and metabolic disorders like diabetes. Overexpression or mutations in Glypican 4 can contribute to oncogenesis as it may lead to aberrant activation of signaling pathways making it relevant in cancers like pancreatic and brain tumors. Additionally Glypican 4's role in metabolic pathways links it to insulin-related pathways emphasizing its connection with metabolic disorders such as Type 2 diabetes. Glypican 4 interacts with proteins such as insulin receptors which further highlights its relevance in these conditions.