Abcam, ab266276, Human TECR (GPSN2) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TECR KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TECR, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The GPSN2 protein also known as Glycoprotein Subunit N2 plays an important role in the regulation of cellular processes. It has an approximate molecular mass of 45 kDa. GPSN2 is expressed in various tissues including the liver kidney and heart reflecting its involvement in different physiological contexts. This protein is involved in the post-translational modification of proteins specifically in N-linked glycosylation which is fundamental for proper protein folding and stability.
Biological function summary
GPSN2 is involved in the biosynthetic machinery as part of the oligosaccharyltransferase complex. This complex is responsible for the transfer of a glycan group to nascent polypeptides in the endoplasmic reticulum. GPSN2 contributes to efficient protein maturation ensuring that proteins acquire their correct conformations and functions. Its presence in this complex indicates important cell functions especially those involving protein processing and trafficking.
Pathways
GPSN2 takes part in the protein glycosylation pathway. This pathway is fundamental for various aspects of cell physiology including cell-cell communication and immune response. GPSN2 interacts with other proteins like STT3A and STT3B which are also components of the oligosaccharyltransferase complex. These interactions highlight its role in the glycosylation pathway influencing cellular homeostasis and signaling.
GPSN2 is associated with congenital disorders of glycosylation (CDG) and liver fibrosis. Structural abnormalities in GPSN2 can lead to CDG characterized by a wide range of developmental and metabolic issues. Additionally GPSN2's involvement in liver fibrosis links it to aberrant protein glycosylation which can exacerbate the progression of this liver disease. In these disorders GPSN2 often interacts with other glycosylation-related proteins such as the aforementioned STT3A further impacting disease development and progression.