Abcam, ab266298, Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MPI KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MPI, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mannose Phosphate Isomerase also known as PMI is an enzyme that catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate. This enzyme with a mass of approximately 42 kDa plays an important role in the metabolism of mannose a type of sugar. Expression of Mannose Phosphate Isomerase occurs in many tissues including liver muscle and fibroblasts where it helps maintain the balance of sugar phosphates needed for various cellular functions.
Biological function summary
This enzyme facilitates the processing of mannose through the biosynthesis pathways of glycoproteins and glycolipids. Mannose Phosphate Isomerase is not part of a larger protein complex but it functions independently to execute its role within the cell. It ensures that cells have adequate mannose-6-phosphate which is essential for proper glycosylation a process necessary for protein folding and stability.
Pathways
Mannose Phosphate Isomerase influences the hexosamine biosynthesis pathway and glycolysis. In the hexosamine biosynthesis pathway it works closely with enzymes such as phosphomannomutase to provide critical components for the production of glycosaminoglycans and glycoproteins. In glycolysis it helps integrate mannose into energy production processes by providing intermediates necessary for glucose metabolism.
Defects in Mannose Phosphate Isomerase can lead to conditions such as congenital disorder of glycosylation type Ib and hypoglycemia-related disorders. This enzyme's malfunction disrupts normal glycosylation which is important for protein function and stability linking it to glycosylation disorders. Connections also exist with phosphomannomutase another enzyme affected in similar glycosylation disorders highlighting its relevance in disease pathology.