Abcam, ab266335, Human RNF167 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RNF167 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RNF167, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF167 also known as RING finger protein 167 is an E3 ubiquitin-protein ligase with a molecular mass of approximately 61 kDa. This protein facilitates the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to specific substrate proteins marking them for degradation via the proteasome. RNF167 is expressed in various tissues with notable presence in the brain indicating its potential importance in neural functions.
Biological function summary
The functions of RNF167 are pivotal in regulating the stability and turnover of membrane proteins which are key to cellular homeostasis. It plays a role in endosomal sorting and trafficking ensuring that proteins reach their proper intracellular destinations. RNF167 forms part of a larger complex involving the ESCRT machinery which is essential for multivesicular body pathway and lysosome degradation processes.
Pathways
RNF167 interacts in significant cellular mechanisms impacting protein degradation and recycling. It participates in the endosomal-lysosomal pathway closely interacting with other proteins like VPS29 which aids in the recycling of transmembrane receptors. The protein also has roles related to the ubiquitin-proteasome system intersecting with pathways involving protein quality control and it functions in concert with proteins like HRS in these processes.
RNF167 has been implicated in neurodegenerative conditions such as Alzheimer's disease where dysregulation of protein degradation pathways contributes to neurofibrillary tangles and plaque formation. Its dysfunction can influence pathways linked to proteins such as APP. Additionally RNF167 associates with cancer where alterations in ubiquitin ligase activity can lead to uncontrolled cell proliferation with links noted to proteins such as MDM2 that regulate the tumor suppressor p53.