Abcam, ab266428, Human TRIP10 (Cip4) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TRIP10 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRIP10, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cip4 also known as Cdc42-interacting protein 4 is a member of the F-BAR family and is involved in cellular processes related to actin dynamics. It has a molecular mass of approximately 70 kDa. Cip4 interacts with Cdc42 a small GTPase to facilitate membrane bending and invagination important for endocytosis and membrane trafficking. This protein is expressed in a variety of tissues including the brain heart and liver reflecting its broad functional significance across different cell types.
Biological function summary
Cip4 plays an essential role in linking membranes to the actin cytoskeleton. It is part of a complex with other proteins like Toca-1 and N-WASP which are important for actin polymerization. This function supports cellular morphogenesis and migration by organizing the actin filaments in response to external signals. Cip4 also contributes to the regulation of cell shape and motility aiding in processes such as development and immune responses by modulating membrane-associated actin assembly.
Pathways
Cip4 participates in pathways including the Cdc42 signaling pathway and endocytosis. In the Cdc42 signaling pathway Cip4 works with proteins like N-WASP and dynamin to facilitate membrane remodeling and cytoskeletal rearrangements impacting processes like cell division and signal transduction. Its involvement in endocytosis influences cargo uptake and intracellular transport linking it to dynamin-dependent endocytic pathways critical for nutrient uptake and receptor internalization.
Cip4 is associated with certain cancers and neurological disorders. Its dysregulation can contribute to tumor progression often involving interactions with proteins like Cdc42 and N-WASP that promote changes in cell adhesion and motility. Additionally abnormalities in Cip4 expression or function may lead to disrupted cell signaling in neurological disorders impacting processes essential for brain development and function. Understanding Cip4's role in these contexts provides insights into potential therapeutic targets for treatment.