Abcam, ab266449, Human MDH2 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MDH2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon 4 and Insertion of the selection cassette in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon 4 and Insertion of the selection cassette in exon 4

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MDH2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MDH2 also known as malate dehydrogenase 2 or mitochondrial malate dehydrogenase is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the conversion of malate to oxaloacetate using NAD+ as a cofactor. It is predominantly expressed in the mitochondria where it plays an important role in cellular respiration. The enzyme enables the malate dehydrogenase reaction which is key for the functioning of the tricarboxylic acid cycle.
Biological function summary
MDH2 participates in the critical process of energy production within the cell. While it does not form a complex itself its activity is intimately connected with other enzymes in mitochondrial energy metabolism. The malate dehydrogenase assay often measures the activity of MDH2 to understand the metabolic status of cells. By facilitating the oxidation of malate MDH2 aids in maintaining the efficiency of the mitochondrial electron transport chain by regenerating NADH.
Pathways
The enzyme is essential in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle MDH2 collaborates with enzymes like citrate synthase and isocitrate dehydrogenase to assist in the conversion of acetyl-CoA into energy-rich molecules. The malate-aspartate shuttle on the other hand involves MDH2 working closely with aspartate transaminase to transfer reducing equivalents into the mitochondria. These pathways highlight MDH2's importance in cellular energy homeostasis.
Mutations or dysregulation in MDH2 have connections to certain metabolic conditions and cancers. For example alterations in MDH2 activity might contribute to conditions like mitochondrial myopathy altering energy metabolism. Moreover MDH2 is associated with NADH-producing enzymes whose dysregulation can support oncogenic pathways in cancer. Understanding these associations helps researchers pursue therapeutic targets that modulate MDH2 activity.