Abcam, ab266463, Human SURF4 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SURF4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SURF4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SURF4 also known as Surfeit locus protein 4 is a membrane protein with a molecular mass of approximately 30 kDa. It functions mechanically as part of the endoplasmic reticulum membrane and plays a role in protein transport. SURF4 facilitates the movement of specific proteins between the endoplasmic reticulum and the Golgi apparatus. This protein exhibits broad expression being present in various tissues including liver and kidney.
Biological function summary
The functions of SURF4 integrate into cellular processes through its involvement in protein secretion and sorting pathways. SURF4 participates as part of a complex that mediates the trafficking of glycoproteins which are essential for numerous cellular activities. The protein assists in the maintenance of quality control within the secretory pathway and ensures that proteins reach their correct destinations which is critical for proper cell function.
Pathways
SURF4 engages actively in processes related to protein transport and glycoprotein trafficking. It participates in the secretory pathway ensuring efficient delivery of proteins to the Golgi apparatus. The protein relates closely to other endoplasmic reticulum proteins like EMC1 which is involved in the coordinated movement and processing of proteins within the cell. SURF4's role intertwines with these components influencing cellular homeostasis and protein synthesis.
SURF4 interlinks with health issues involving protein misfolding and secretion defects. Mutations or dysregulation can lead to conditions like congenital disorders of glycosylation characterized by defects in glycoprotein synthesis and processing. Dysregulation of SURF4 activity may also impact hepatic steatosis as SURF4 is implicated in lipid metabolism. The interaction with proteins like SEC61 which aids in protein translocation highlights its importance in maintaining cellular balance and reducing disease risk.