Abcam, ab266528, Human GNB2 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
GNB2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-GNB2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GNB2 also known as Guanine Nucleotide Binding Protein Subunit Beta 2 is part of the G protein family. It has a molecular mass of approximately 38 kDa. GNB2 functions mechanically by acting as a modulator or transducer in various signaling pathways. This protein is expressed in a wide range of tissues including the brain heart and muscles. Its role involves interacting with alpha and gamma subunits to form a heterotrimeric G protein complex that is important for signal transduction.
Biological function summary
In the context of cellular processes GNB2 is involved in transmitting signals from activated G protein-coupled receptors to different intracellular effectors. It forms an essential part of the G protein complex which plays a role in facilitating the exchange of GDP for GTP on the alpha subunit. The activation of the G protein complex enables downstream signaling that governs multiple physiological responses such as sensory perception and cellular communication.
Pathways
GNB2 is heavily involved in pathways related to signal transduction and sensory perception. It plays a significant role within the GPCR (G protein-coupled receptor) signaling pathway an important pathway involved in transmitting signals from diverse stimuli outside a cell to its interior. In this context GNB2 interacts with related proteins such as GNAI2 contributing to the regulation of ion channels adenylyl cyclases and other effectors. This connectivity enables precise cellular responses to external signals.
Research indicates that GNB2 is associated with cardiac arrhythmias and neuropsychiatric disorders. It has connections to other proteins like GNAO1 in the context of these conditions. Mutations or dysregulation of GNB2 can disrupt normal signaling processes leading to these diseases. Understanding the role of GNB2 in these disorders highlights potential therapeutic targets for developing new interventions.