Abcam, ab266578, Human STRAP (Unrip) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
STRAP KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 1 bp insertion in exon 3 and 5 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 1 bp insertion in exon 3 and 5 bp deletion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-STRAP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Unrip also known as NRIP (nuclear receptor interaction protein) is a nuclear protein with a molecular mass of approximately 64 kDa. Researchers have identified its expression mainly in the nucleus across various tissues. Mechanically Unrip functions as a factor interacting with nuclear hormone receptors. It regulates transcriptional activity through direct contact with these receptors modulating gene expression. This interaction plays a role in how cells respond to specific hormones and signals.
Biological function summary
Unrip contributes significantly to the regulation of gene expression by associating with other nuclear coregulators. It is part of a larger protein complex that includes importin beta and RIC1/RAB1. This complex facilitates the proper localization and functioning of nuclear hormone receptors affecting the expression of target genes. Unrip's involvement with such complexes illustrates its integral function in transcriptional regulation and cellular signaling pathways.
Pathways
Unrip integrates into essential signaling networks particularly the nuclear receptor signaling and coactivator pathways. In these pathways Unrip modulates the activity of nuclear receptors like estrogen receptor alpha and glucocorticoid receptor affecting cellular responses to steroid hormones. Unrip interacts with proteins such as SRC-1 and GRIP1 within these pathways influencing gene expression profiles related to growth differentiation and metabolic processes.
Unrip's interactions impact conditions including cancer and hormone-related disorders. Its role in modulating estrogen and glucocorticoid receptors links it to breast cancer progression where these pathways are dysregulated. Additionally Unrip's involvement in nuclear receptor signaling ties it to metabolic disorders like type 2 diabetes. Through the alteration of receptor pathways Unrip affects proteins like PPARγ demonstrating its relevance in disease mechanisms and therapeutic research.