Abcam, ab266638, Human NOL7 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NOL7 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NOL7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nucleolar Protein 7 commonly known as NOL7 is a human protein with a mass of approximately 26 kDa. NOL7 is mainly found in the nucleolus of cells which indicates its potential role in ribosomal RNA processing or ribosome assembly. As a nucleolar protein NOL7 expression occurs across various tissues providing a widespread substrate for its biological functions.
Biological function summary
The role of NOL7 involves regulation of cellular growth and proliferation. It acts potentially as a tumor suppressor contributing to the control of angiogenesis and maintaining cell cycle progression. NOL7 forms part of larger nucleolar complexes participating in ribonucleoprotein formation needed for effective cellular operations. Its presence and activity help maintain normal cellular and tissue homeostasis.
Pathways
NOL7 influences pathways involved in angiogenesis and cellular proliferation. It interacts directly with certain key proteins like Vascular Endothelial Growth Factor (VEGF) inhibiting its excessive expression and therefore functioning as part of the angiogenic pathway. NOL7's activity aids the maintenance of balanced angiogenic signals helping to regulate vascular development within tissues.
The expression and functionality of NOL7 have connections to cancer and cardiovascular diseases. NOL7's potential tumor-suppressive properties relate it to various cancer types where its dysregulation may contribute to uncontrolled cell growth. In the context of cardiovascular disease NOL7 interacts with VEGF playing a role in the pathological neovascularization processes. Efforts to understand NOL7 could provide insights into new therapeutic strategies for diseases involving abnormal angiogenesis.