Abcam, ab266685, Human UQCC2 (MNF1) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
UQCC2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-UQCC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MNF1 also known as mitonuclear factor 1 is a transcription factor with a molecular mass of approximately 40 kDa. It localizes mainly in the mitochondria of cells within tissues with high-energy demands like muscle and nerve cells. MNF1 binds to specific DNA sequences impacting the transcription process by modulating the expression of genes involved in mitochondrial function.
Biological function summary
MNF1 impacts cellular energy metabolism and oxidative phosphorylation. It forms part of a larger mitochondrial transcription complex which also includes other transcription factors and polymerases. MNF1's main role lies in regulating the replication and transcription of mitochondrial DNA which is essential for maintaining mitochondrial integrity and function.
Pathways
MNF1 participates in the mitochondrial biogenesis and cellular respiration pathways. It interacts closely with proteins such as PGC-1α an important regulator of energy metabolism that helps to enhance mitochondrial gene expression. MNF1's actions promote efficient mitochondrial function linking it to broader cellular energy management processes.
Alterations in MNF1 levels have associations with mitochondrial disorders and neurodegenerative diseases. Mutations or malfunctions in MNF1 can lead to disruptions in the normal operation of mitochondrial functions potentially linking it to conditions such as mitochondrial myopathies. MNF1 also interacts with other key mitochondrial proteins making it a potential target for understanding the molecular basis of these conditions.