Abcam, ab266705, Human TRMT1 (TRM1) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TRMT1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 6. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 6

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRMT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRM1 also known as transfer RNA methyltransferase 1 is an enzyme responsible for methylating the N2 position of guanine in tRNA molecules. It has a molecular mass of approximately 49 kDa. TRM1 mostly resides in the mitochondria but can also be found in the cytoplasm. Its presence ensures proper methylation of tRNA influencing efficient and accurate protein translation.
Biological function summary
The methylation activity of TRM1 plays a role in maintaining the stability and function of tRNA molecules. As part of the cellular machinery TRM1 does not function alone; it often works together with other tRNA-modifying enzymes to ensure the fidelity of protein synthesis. This modification protects tRNA's structure facilitates proper folding and enhances interaction with ribosomal components.
Pathways
TRM1 functions significantly impact protein synthesis pathways. The enzyme is instrumental in the translation process by ensuring that tRNA molecules are correctly modified for protein assembly. TRM1 operates alongside other proteins such as TRM10 and TRMT5 within these modification pathways influencing the efficiency and accuracy of translation. It supports processes like tRNA maturation and ribosomal activity.
Alterations in TRM1 activity can relate to mitochondrial pathologies. Mutations or deficiencies in TRM1 can disrupt mitochondrial function leading to disorders such as mitochondrial myopathy. Research also connects TRM1 aberrations to neurodegenerative diseases where it may interact with proteins like PINK1 highlighting its role in neuroprotective responses. The efficiency of TRM1 in performing its tasks is vital as any dysfunction can result in profound cellular consequences affecting human health.