Abcam, ab266818, Human ANP32B (PHAPI2/APRIL) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
ANP32B KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ANP32B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHAPI2 also known as APRIL is a protein with various roles in cellular mechanisms. The protein has a molecular mass of around 32 kDa and it is found in many tissues including the spleen liver and lymph nodes. PHAPI2 acts as part of the tumor necrosis factor ligand superfamily where it influences cell proliferation and survival. This protein interacts with receptors such as BCMA and TACI impacting immune system functions.
Biological function summary
PHAPI2 regulates immune system processes by playing a role in B cell development and activity. It functions as part of larger protein complexes binding with proteins such as BCMA to influence signaling pathways. The interactions within these complexes help modulate processes critical for proper immune response and maintenance of immune system homeostasis.
Pathways
PHAPI2 is deeply involved in both the NF-kB signaling pathway and the PI3K/AKT pathway. These pathways are essential for cellular growth survival and proliferation. Through its involvement PHAPI2 also connects with proteins like NF-kB and AKT1 which are key regulatory proteins within these pathways. This connection enables PHAPI2 to act as a node integrating signals for various cellular responses.
PHAPI2 associations include autoimmune diseases and cancer. Aberrant expression or mutations in PHAPI2 are linked to conditions like systemic lupus erythematosus and certain B cell malignancies. Through these disorders PHAPI2 interacts with proteins such as BAFF and CD40 which are important for disease progression and immune dysregulation. This makes PHAPI2 a potential therapeutic target for intervention in these conditions.