Abcam, ab266821, Human PLS3 (T Plastin/PLS3) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PLS3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 5 bp deletion in exon 5. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 5 bp deletion in exon 5

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PLS3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The T Plastin also known as PLS3 is a protein with a mass of around 70 kDa. This protein belongs to the plastin family and another popular alias is plastine. T Plastin participates in cellular mechanics by bundling actin filaments which is essential for maintaining cell structure and motility. It occurs in various tissues but is highly expressed in the intestines testis and other cells with rapid movement needs like immune cells. Its presence supports functions needing dynamic rearrangement of the cytoskeleton.
Biological function summary
T Plastin plays significant roles in several cellular activities. It stabilizes the actin cytoskeleton which impacts cell division signal transduction and cellular interactions. T Plastin is not part of a multi-protein complex but works closely with actin and other actin-binding proteins to regulate cytoskeletal organization. By supporting these functions T Plastin indirectly influences cell shape and intracellular transport mechanisms.
Pathways
T Plastin integrates into the actin dynamics pathways important for cytoskeletal reorganization. It works alongside proteins such as cofilin and profilin which also regulate actin dynamics. Another important pathway includes wound healing where T Plastin's actin-bundling function assists in cell migration and tissue regeneration. In these pathways its role ensures efficient cellular responses to environmental changes.
Defects in T Plastin have been linked to osteoporosis and some forms of cancers. In osteoporosis improper expression of T Plastin affects bone density regulation partly due to its influence on osteoclast function. Cancers such as colorectal cancer exhibit altered T Plastin levels which may impact metastasis due to changes in cell motility. In these contexts T Plastin's interaction with other proteins like integrins highlights its importance in controlling cell adhesion and migration associated with disease progress.