Abcam, ab266889, Human POR (Cytochrome P450 Reductase) knockout HCT116 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
POR KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 4,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-POR, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytochrome P450 reductase also known as NADPH--cytochrome P450 reductase (CPR) is an enzyme important in electron transfer. It has a mass of approximately 77 kDa and is responsible for transferring electrons from NADPH to cytochrome P450 enzymes supporting their function. The enzyme is predominantly expressed in the endoplasmic reticulum of liver cells and other tissues where detoxification processes occur. Cytochrome reductase as it is sometimes called plays a fundamental role in the metabolism of drugs and the synthesis of cholesterol steroids and other lipids.
Biological function summary
CPR facilitates electron donation to all microsomal cytochrome P450 enzymes which are involved in a variety of oxidative reactions. This protein is essential for P450 activity assay as it is part of a multienzyme complex that metabolizes exogenous and endogenous compounds efficiently. Without cytochrome P450 reductase the catalytic activity of cytochrome P450 enzymes becomes impaired affecting metabolizing mechanisms for various substances and having systemic biological impacts.
Pathways
Cytochrome P450 reductase directly participates in drug metabolism and steroid biosynthesis pathways. It together with the cytochrome P450 enzymes metabolizes drugs to make them more water-soluble for excretion. The proteins 3 beta-hydroxysteroid dehydrogenase and CYP3A4 are some of the related components within these pathways working in conjunction to achieve metabolic homeostasis. P450 reductase assays are often utilized to study these pathways further understanding the biological roles and interactions of these proteins.
CPR has a significant connection to conditions like congenital adrenal hyperplasia and prostate cancer. Mutations or dysregulations in cytochrome P450 reductase can lead to accumulated steroid precursors and disrupted steroid hormone synthesis contributing to these disorders. The relationship with adrenal enzyme 21-hydroxylase and the androgen receptor illustrates the pathway complexities and the clinical relevance of cytochrome P450 reductase. Understanding these connections highlights the importance of CPR in maintaining normal physiological functions and how its abnormalities can lead to serious health issues.