Abcam, ab266892, Human HELLS (SMARCA6) knockout HCT116 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
HELLS KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-HELLS, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMARCA6 also known as SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6 is a protein with a molecular weight of approximately 170 kDa. It functions as an ATPase and is an important component of chromatin remodeling complexes. SMARCA6 plays a role in modifying chromatin structure through ATP hydrolysis which influences gene expression. This protein is expressed in various tissues and its expression can be detected across different cell types including those involved in blood functions.
Biological function summary
SMARCA6 influences gene expression by modifying the chromatin structure which allows transcription factors to access DNA. It is a part of the SWI/SNF chromatin remodeling complex critical for regulating a wide range of genes involved in development and cell cycle. Through the remodeling of chromatin SMARCA6 controls access to specific genomic regions and modulates transcriptional activation or repression therefore influencing cellular differentiation and proliferation.
Pathways
SMARCA6 participates in pathways involved in chromatin organization and gene regulation. It engages in the Wnt signaling pathway interacting with proteins such as BAF155 and BAF170 to modulate transcriptional outcomes. SMARCA6's activity in these pathways is essential for cellular processes impacting how cells respond to growth signals and maintain homeostasis.
Aberrations in SMARCA6 function or expression can lead to complications such as cancer and neurodevelopmental disorders. It has been linked to leukemia where chromatin remodeling anomalies disrupt normal gene expression patterns. In such contexts SMARCA6 interacts with proteins like BAF57 affecting the regulation of oncogenes and tumor suppressors. Altered SMARCA6 activity in these diseases highlights its role in maintaining genomic stability and proper cell function.