Abcam, ab267077, Human IFI6 knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
IFI6 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-IFI6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IFI6 also known as Interferon Alpha-Inducible Protein 6 is a 130-amino acid protein with a mass of approximately 14.7 kDa. This protein locates itself in the mitochondrial outer membrane and is expressed abundantly in tissues such as the liver muscle and kidney. The expression of IFI6 increases in response to interferon treatment especially interferons alpha and beta.
Biological function summary
IFI6 plays a role in apoptotic resistance mechanisms helping cells to avoid programmed death under stress conditions. This protein does not function as part of any large complex but operates independently to regulate apoptosis by controlling the mitochondrial membrane potential. Its involvement in cellular growth and immune response proves significant especially in viral infections where it modulates the cell's response to viral replication.
Pathways
IFI6 integrates into the interferon signaling pathway which is important in defending against viral infections. It collaborates with other proteins such as STAT2 and IRF9 to activate immune responses. Moreover IFI6 influences the apoptosis pathway where it interacts with anti-apoptotic proteins like Bcl-2 to support cell survival under certain conditions.
IFI6 is implicated mainly in viral infections and cancer. Its expression levels can affect viral persistence during infections such as hepatitis C. In cancer IFI6 has been observed in the context of melanoma where it interacts with proteins like Bcl-2 to potentially alter cancer cell survival and resistance to therapies. Understanding IFI6's role may hold importance for developing targeted treatments in such conditions.