Abcam, ab267089, Human IFI27 knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
IFI27 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 146 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 146 bp deletion in exon 4,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-IFI27, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IFI27 also known as Interferon alpha-inducible protein 27 is a small protein with a molecular mass of around 12 kilodaltons. This protein is expressed predominantly in response to type I interferons and some other stimuli. It mainly appears in tissues like the skin and liver which are involved in immune responses. IFI27 localizes to the mitochondria suggesting its role in regulating cellular apoptosis.
Biological function summary
IFI27 plays a significant role in mediating apoptosis the programmed cell death essential for normal cellular turnover and homeostasis. Although it does not typically form part of a larger protein complex its induction by interferons implicates it in antiviral defense mechanisms. Its expression can lead to changes in mitochondrial membrane potential ultimately affecting cell survival.
Pathways
IFI27 participates in important pathways related to immunity and apoptosis. Particularly it engages in the interferon signaling pathway influencing the cellular defense against viral infections. It interacts with other proteins like STAT1 and STAT2 which are key transcription factors in the interferon signaling cascade modulating downstream antiviral responses.
IFI27 has associations with chronic liver diseases and certain autoimmune conditions such as systemic lupus erythematosus (SLE). Its elevated expression often indicates an active immune response or cellular stress. IFI27's expression relates to IFI6 another interferon-inducible protein both of which are implicated in the cellular responses observed in these conditions. Understanding IFI27's role may help in developing therapeutic strategies for targeting immune-related disorders.