Abcam, ab267164, Human IFI44L knockout A549 cell line

Size: 1000000Cells / vial
IFI44L KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 2 and 5 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 2 and 5 bp deletion in exon 2,
Disease:Carcinoma

Product details:
Recommended control:
Human wild-type A549 cell line (ab255450). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-IFI44L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The interferon-induced protein 44-like (IFI44L) is a protein encoded by the IFI44L gene. It is also known as IFI44L protein or sometimes simply IFI44L. This protein has a molecular mass of around 49 kDa. IFI44L is broadly expressed in many tissues with higher expression in blood and spleen. Mechanically IFI44L contributes to the antiviral response by being induced by type I and type II interferons. Its exact mechanism of action involves modulation in response to viral infection although full details of how it works are still not completely understood.
Biological function summary
IFI44L plays an active role in the body's defense against viral infections. It participates as part of the broader immune response. While not being recognized as a classic element of a large protein complex it interacts with cellular machinery responsible for immune regulation and interferon signaling. This protein contributes towards the establishment of an antiviral state in affected cells through mechanisms which are yet to be completely defined. Its inducible nature makes it an important part of how cells respond to foreign viral components.
Pathways
This target connects to the interferon signaling pathway which is important for antiviral defenses. IFI44L plays a significant role in the broader cytokine signaling pathways particularly those related to type I interferon responses. It interacts with proteins such as MX1 and IFIT1 which are important elements of the interfacing network that responds to viral challenges in the cell. These relationships help in sustaining a robust antiviral state within the host.
IFI44L is linked to conditions like systemic lupus erythematosus (SLE) and viral infections such as hepatitis C. This can be due to its regulatory role in immune responses and interferon signaling. In SLE where interferon activity is often dysregulated IFI44L expression can be elevated. It also connects with proteins like IFI27 and ISG15 which are similarly upregulated in response to viral infections and are implicated in the pathogenesis of these diseases. Understanding IFI44L's function and interactions further aids in exploring therapeutic approaches for these conditions.