Abcam, ab267189, Human LGALS9C knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
LGALS9C KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon 3 and 4 bp deletion in exon 3 and 4 bp insertion in exon 3 and 8 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon 3 and 4 bp deletion in exon 3 and 4 bp insertion in exon 3 and 8 bp deletion in exon 3,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LGALS9C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LGALS9C also known as Galectin-9c belongs to the galectin family which mediates cell-cell interactions cell-matrix adhesion and apoptotic pathways. This protein has a molecular mass of approximately 36 kDa. You can find it expressed in various tissues with notable presence in immune cells suggesting a role in immune system regulation. Researchers often associate other names such as Gal-9S and Galectin-9c Isoform with this protein indicating its various isoforms adapt functions in different cells.
Biological function summary
LGALS9C influences immune response modulation highlighting its presence in immune cell function. It interacts with biologically significant partners often forming a complex with receptors like Tim-3 on T cells. This interaction can either promote cell death in certain contexts or drive anti-inflammatory responses. The involvement of LGALS9C in regulating the survival and function of these cells underlines its importance in maintaining homeostasis within the immune system.
Pathways
LGALS9C plays significant roles within immune signaling pathways including the Tim-3/Galectin-9 pathway. It also intersects with the apoptosis regulatory pathway influencing cellular outcomes during immune responses. LGALS9C interacts with proteins such as Tim-3 and PD-1 which share pathways that regulate immune responses and tolerance. The modulation of immune checkpoints places LGALS9C within a network of pathways that could influence therapy in chronic inflammation and cancer.
LGALS9C shows relevance to autoimmune diseases and cancer. It has been observed to dysregulate inflammatory responses contributing to conditions like systemic lupus erythematosus (SLE). During cancer progression LGALS9C can affect tumor microenvironment by interacting with proteins such as Tim-3 and influencing immune evasion mechanisms. These connections make LGALS9C a target of interest for therapeutic interventions aiming to modulate immune responses in disease treatment.