Abcam, ab26721, Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade

Size: 100µg
Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721) is a rabbit polyclonal antibody detecting RNA polymerase II CTD repeat YSPTSPS in Western Blot, IP, IHC-P, ICC/IF, ChIP . Suitable for Human . - Over 50 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IP, IHC-P, ChIPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Replenishment batches of our polyclonal antibody, ab26721 are tested in ChIP. Previous batches were additionally validated in ICC/IF, IHC-P, IP and WB. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab238146.

Product details:
Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721) is a rabbit polyclonal antibody and is validated for use in ChIP, ICC/IF, IHC-P, IP, WB in arabidopsis thaliana, caenorhabditis elegans, drosophila melanogaster, human, mouse, rat, saccharomyces cerevisiae, schizosaccharomyces pombe, xenopus laevis, zebrafish samples.
Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721) has been cited over 55 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721) has high sensitivity and specificity.
Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721) has 8 independent reviews from customers.
Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721) specifically detects RNA polymerase II CTD repeat YSPTSPS (UniProt ID: P24928; Molecular weight: 217kDa) and is sold in 100 µg selling sizes.
RNA polymerase II CTD repeat YSPTSPS is essential for transcription regulation and gene expression. Utilizing our highly specific RNA polymerase II CTD repeat YSPTSPS antibody can significantly enhance the detection and study of this modification. Research indicates that phosphorylation of the CTD repeat YSPTSPS is crucial for the transition from transcription initiation to elongation, impacting various cellular processes and disease states. Phosphorylation of RNA polymerase II at the CTD repeat YSPTSPS is critical for proper mRNA synthesis and processing. Abnormal phosphorylation patterns are linked to transcriptional dysregulation in cancer, pediatric gliomas, chondroblastomas, and neurodevelopmental syndromes. Studying these modifications can provide insights into potential therapeutic targets for various conditions.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNA polymerase II CTD repeat YSPTSPS also known as the C-terminal domain of RNA polymerase II is a critical component of the RNA polymerase II enzyme commonly referred to as pol II. This domain is characterized by the repetitive sequence YSPTSPS which plays a significant role in the regulation of transcription. The mass of RNA polymerase II including its CTD varies but is essential for its function in gene expression. RNA polymerase II with the CTD is expressed in the nucleus of eukaryotic cells where it orchestrates the transcription of DNA into mRNA.
Biological function summary
RNA polymerase II CTD repeat YSPTSPS is essential for the transcription progression from initiation to termination. It is part of the large RNA polymerase II complex interacting with various transcription factors and enzymes necessary for RNA processing. The phosphorylation state of the CTD particularly on serine residues regulates interactions with splicing machinery and other RNA processing factors. This modulation ensures the coupling between transcription and RNA processing events controlling mRNA synthesis and maturation.
Pathways
RNA polymerase II CTD repeat YSPTSPS is important in the mRNA synthesis pathway specifically in transcriptional regulation and processing of nascent RNA transcripts. It interacts with proteins such as the transcription factors TFIIH and TFIIB which aid in promoter recognition and open complex formation. The CTD's dynamic phosphorylation pattern allows integration into multiple cellular pathways most importantly connecting transcription with RNA splicing and transport pathways.
Abnormal function or mutations in RNA polymerase II CTD repeat YSPTSPS associate with diseases such as transcription-related syndromes and certain cancers. Deficient CTD phosphorylation can lead to improper mRNA processing resulting in neural developmental disorders. Additionally its interaction with proteins like CDK7 which phosphorylates the CTD links it to tumors where transcriptional dysregulation is a hallmark. Understanding the CTD's role in these diseases provides insight into therapeutic targets and strategies for intervention.