Abcam, ab267238, Human SPAST (Spastin) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SPAST KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 4 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 4 bp deletion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SPAST, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Spastin also known as SPG4 is a microtubule-severing ATPase that plays an important role in cellular mechanics by cutting microtubules in cells. It weighs approximately 68 kDa and is present mainly in neural tissue but can also be found in other tissues. The protein's localization includes the cytoplasm and is associated with centrosomes and subcellular regions with dense microtubule networks.
Biological function summary
Spastin participates in regulating microtubule dynamics. It plays a critical role in axonal growth and maintenance by modulating microtubule lengths and branching. Spastin does not function alone; it forms part of the AAA protein family functioning in significant processes like neurogenesis and neural maintenance. Its activity affects neural cell structure and transport by influencing cytoskeletal rearrangement.
Pathways
Any disturbances in Spastin function can impact the microtubule dynamics pathway and the axonal transport pathway. Spastin interacts closely with proteins such as Tubulin and Katanin which are also involved in microtubule severing and organization. Proper Spastin function is necessary to maintain intracellular transport and signal transduction critical for neural cell health and function.
Alterations in Spastin activity lead to neurodegenerative conditions like Hereditary Spastic Paraplegia (HSP). This disorder often relates to mutations in the SPG4 gene which encodes Spastin affecting motor neuron functions. In HSP disrupted Spastin function correlates with deficiencies in proteins such as Tubulin exacerbating neuroaxonal deficits and influencing disease progression.