Abcam, ab267300, Human NFATC3 (NFAT4) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NFATC3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NFATC3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein NFAT4 also known as NF-ATc3 or NFATc3 is a transcription factor with a molecular mass of approximately 140 kDa. It is part of the nuclear factor of activated T-cells (NFAT) family. This protein primarily localizes in the cytoplasm and nucleus and is especially abundant in immune cells like T-cells and various other cell types including neuronal tissues and cardiac cells.
Biological function summary
NFAT4/NF-ATc3 acts as a regulator of gene expression by binding to DNA in the nucleus. It forms part of the NFAT complex working with other members of this family to regulate genes involved in cell differentiation and development. In immune cells this protein plays a role in mediating responses to external signals influencing cytokine production and aiding the regulation of immune responses. Other tissues including neurons and cardiac cells also rely on NFAT4 for specific signaling functions related to cell growth and survival.
Pathways
NFAT4/NF-ATc3 is involved in key signaling mechanisms notably the calcium signaling pathway. This pathway works closely with other proteins such as calcineurin which dephosphorylates NFAT proteins leading to their activation and nuclear translocation. Additionally interactions within the Ras/MAPK pathway highlight NFAT4's role in cell proliferation and differentiation processes interconnecting with various signaling proteins that drive these pathways.
Abnormal NFAT4 activity links to immune-related conditions and cardiac hypertrophy. Its dysregulation associates with autoimmune diseases wherein it affects T-cell activation and inflammation. In cardiac disorders excessive activation of NFAT4 in conjunction with calcineurin contributes to maladaptive cardiac growth presenting a potential therapeutic target for hypertrophic heart conditions. The understanding of NFAT4's involvement in these diseases emphasizes its significance in developing targeted treatments.