Abcam, ab267568, Human NrCAM ELISA Kit

Size: 1 x 96Tests
Human NrCAM ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human NrCAM in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture media, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.3 ng/mL,
Range:0.328 - 80 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human NrCAM ELISA Kit is designed for the quantitative determination of NRCAM in cell culture supernatants, plasma and serum samples.
ΔNote:
Human NrCAM concentration is low in normal serum and plasma samples and may not be detectable in this assay.
This assay employs an antibody specific for human NrCAM coated on a 96-well plate. Standards and samples are pipetted into the wells and NrCAM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human NrCAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NrCAM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NrCAM also known as Neuronal Cell Adhesion Molecule is a protein involved in cell-cell adhesion. It is part of the immunoglobulin superfamily and has a molecular weight of approximately 135 kDa. NrCAM is expressed mainly in the nervous system including areas like the brain and spinal cord. It plays a role in the development and maintenance of neural connections acting as a conduit between neurons.
Biological function summary
NrCAM functions in neural development and synaptic plasticity. It is not only a standalone protein but forms complexes with other adhesion molecules to maintain neural circuit integrity. Through its interactions NrCAM supports processes like axon guidance and dendritic spine formation. It also participates in signaling pathways that regulate these neural processes contributing to the dynamic environment necessary for brain development and function.
Pathways
NrCAM is involved in the formation of neural networks and cellular signaling cascades. It links to pathways such as the axon guidance pathway and neuronal signaling cascade. Through these routes it interacts with proteins like L1CAM and contactin which are pivotal in promoting correct neural patterning. Together these proteins facilitate the direction and bonding between neurons ensuring the establishment of proper neural connections.
NrCAM has associations with neurological conditions like autism spectrum disorder and schizophrenia. Research indicates that alterations in NrCAM expression or function may contribute to the symptoms observed in these disorders. It links with proteins such as DISC1 in schizophrenia context affecting pathways that influence brain connectivity and cognitive function. Understanding NrCAM's role could lead to advancements in therapeutic strategies for these conditions.