Abcam, ab267571, Human PGRPs ELISA Kit

Size: 1 x 96Tests
Human PGRPs ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PGRPs in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 6 pg/mL,
Range:6 - 1500 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human PGRPs ELISA Kit is designed for the quantitative determination of PGRPs in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human PGRPs coated on a 96-well plate. Standards and samples are pipetted into the wells and PGRPs present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PGRPs antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PGRPs bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA antibody pair detects human PGRPs, less than 0.05% cross-reactivity with human PGRP-1 beta and mouse PGRPs is observed.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Peptidoglycan Recognition Proteins (PGRPs) are pattern recognition receptors known for their role in innate immunity. Some alternate names for PGRPs include PGLYRPs. These proteins identify bacterial peptidoglycans triggering immune responses. PGRPs have varying molecular masses that range typically between 20 to 25 kDa. Expression of PGRPs occurs in several tissues including skin eyes liver and throughout the gastrointestinal tract.
Biological function summary
Functioning in innate immunity PGRPs bind to bacterial cell wall components such as peptidoglycan and lipopolysaccharides. When PGRPs detect these molecules they can activate immune responses to neutralize pathogens. Some PGRPs can also act as amidases breaking down peptidoglycans. PGRPs do not usually act alone; they are part of complexes with other immune proteins to enhance pathogen recognition and response.
Pathways
PGRPs play significant roles in the immune pathway and inflammatory signaling pathways. They interact with the toll-like receptor pathways to mediate responses to bacterial infections. PGRPs also link with Nod proteins to modulate the immune response upon detecting peptidoglycans. These pathways help coordinate an effective defense against infection.
PGRPs’ malfunction or dysregulation links with inflammatory bowel disease and psoriasis. Altered PGRP levels correlate with changes in the gut flora contributing to inflammatory bowel disease. In psoriasis PGRPs’ improper function may exacerbate skin inflammation because they interact with proteins like IL-6 and TNF-alpha. Such interactions highlight their potential as therapeutic targets for these conditions.