Abcam, ab267598, Human DAND5 ELISA Kit

Size: 1 x 96Tests
Human DAND5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human DAND5 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.2 ng/mL,
Range:0.205 - 50 ng/mL,
Assay Platform:Microplate

Product details:
Human DAND5 ELISA Kit is designed for the quantitative determination of DAND5 (COCO) in cell culture supernatants, plasma and serum samples.
Δ Note:
Human DAND5 concentration is low in normal serum and plasma samples and may not be detectable in this assay.
This assay employs an antibody specific for human DAND5 coated on a 96-well plate. Standards and samples are pipetted into the wells and DAND5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human DAND5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DAND5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
No cross-reactivity with human ActivinA, BMP4, DAN or mouse DAND5 is observed.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C