Abcam, ab267603, Human Legumain ELISA Kit

Size: 1 x 96Tests
Human Legumain ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Legumain in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 28.7 pg/mL,
Range:28.67 - 7000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Legumain ELISA Kit is designed for the quantitative determination of Legumain in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Legumain coated on a 96-well plate. Standards and samples are pipetted into the wells and Legumain present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Legumain antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Legumain bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human 2B4 (CD244), ADAM-9, Angiopoietin-2, APRIL, BMP-2, BMP-9, C5a, Cathepsin L, CD200, CD97, Chemerin, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha / betaR2, IGF-2, IGF-2 R, IL-1 R6 (IL-1 Rrp2), IL-24, IL-33 (IL-1 F11), Kallikrein 14.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Legumain also known as asparaginyl endopeptidase is an enzyme that plays a mechanical role as a protease. This enzyme has a molecular mass of approximately 56 kDa and it functions by catalyzing the hydrolysis of asparagine residues. Legumain is expressed in various tissues with higher levels observed in kidneys placenta and liver. Its proteolytic activity is essential for the breakdown of intracellular proteins within lysosomes and endosomal compartments.
Biological function summary
Legumain contributes to protein degradation and antigen processing. It does not operate alone as it is part of larger protein complexes which enhance its stability and function. Through its role in proteolysis legumain influences cellular processes like maturation of endolysosomal enzymes and processing of proenzymes. Additionally it assists in activating other proteases signifying its critical position in maintaining cellular proteostasis.
Pathways
Legumain participates in the regulation of immune responses and cellular homeostasis. It plays a significant role in the lysosomal pathway where it partners with cathepsins to assist in the degradation of proteins. Additionally legumain influences the MHC class II pathway by contributing to the cleavage of invariant chain aiding antigen presentation. Its interaction with cathepsins and the impact on antigen processing indicates its necessity in immune system functionality.
Legumain has been linked to disorders such as cancer and neurodegeneration. In cancer elevated legumain levels correlate with tumor invasiveness and metastasis especially in association with proteins like matrix metalloproteinases. Meanwhile in neurodegenerative conditions its dysregulation may contribute to abnormal protein accumulation and synaptic dysfunction. Understanding legumain's role and interactions can guide therapeutic strategies for these diseases.