Abcam, ab267800, Mouse NT-ProANP ELISA Kit

Size: 1 x 96Tests / 10 x 96Tests
Mouse NT-ProANP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse NT-ProANP with a sensitivity of 90.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Cell culture supernatant, EDTA Plasma, Citrate plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 90.7 pg/mL,
Range:125 - 8000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse NT-ProANP ELISA Kit ab267800 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse NT-ProANP in cell culture media, cell culture supernatant, EDTA plasma, heparin plasma. This SimpleStep sensitivity is 43.4 pg/mL.
How the assay works
Mouse NT-ProANP SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse NT-ProANP ELISA Kit ab267800 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-proANP antibody [EPR22091-19] - BSA and Azide free (Capture)
ab269383
- Anti-proANP antibody [EPR22051-108] - BSA and Azide free (Detector)
ab269383
Mouse NT-ProANP is the propeptide portion of the 129 amino acids long Atrial Natriuretic Peptide (ANP) preproprotein. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. The ANP proprotein is cleaved by serine protease corin to produce the functional hormone, atrial natriuretic factor (ANF). ANF circulates in plasma; it is involved in cardiovascular homeostasis through the regulation of natriuresis, diuresis, and vasodilation directed toward reduction of body fluid and blood pressure regulation. NT-ProANP circulates at low levels at the early stages of hypertension and increases during chronic heart failure. Mouse NT-ProANP is 94% identical to rat NT-ProANP on amino acid level. This kit recognizes mouse NT-ProANP protein (amino acids 25 – 122 of Uniprot # P05125). This kit does not recognize mouse ANP proprotein (amino acids 25 – 152 of Uniprot # P05125).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The proANP also known as N-terminal pro-atrial natriuretic peptide (NT-proANP) is a biochemical precursor involved in the synthesis of atrial natriuretic peptide (ANP). This peptide is expressed mainly in the cardiac atrial myocytes. proANP has a molecular mass of approximately 28 kDa. The heart releases it in response to atrial stretch due to increased blood volume assisting the body in managing vascular homeostasis.
Biological function summary
NT-proANP participates in fluid balance and blood pressure regulation. It is part of a complex system involved in natriuresis which promotes sodium excretion via urine therefore reducing blood volume and pressure. ANP the active hormone plays a large role in vasodilation contributing to cardiovascular health by decreasing peripheral resistance and reducing cardiac workload.
Pathways
ProANP's role extends into the renin-angiotensin-aldosterone system (RAAS) and the natriuretic peptide system. Within these pathways NT-proANP acts to counterbalance the effects of angiotensin II and aldosterone which increase blood pressure and fluid retention. It has a significant relationship with protein NPPB also known as brain natriuretic peptide (BNP) both of which function to support cardiovascular stability and electrolyte balance.
NT-proANP has connections to conditions like heart failure and hypertension. Elevated levels of NT-proANP may indicate heart failure as the heart releases more peptides in response to increased atrial pressure. NT-proANP's relationship with NPPB is clinically useful as both markers are used to diagnose and monitor heart failure. Their combined expression offers insights into cardiac function and the efficacy of therapeutic interventions.