Abcam, ab268650, Recombinant human B19 protein (Active)

Size: 5µg
Recombinant human B19 protein (Active) is a Human Full Length protein, in the 1 to 714 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific actvity of ab268650 was determined to be 2 nmol/min/mg in a kinase assay using MBP ubstrate.,
Accession:P57058,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Human parvovirus B19 also known simply as B19 is a small DNA virus with a linear genome and a non-enveloped capsid. The virus itself is approximately 5.6 kilodaltons in mass. B19 targets and infects erythroid progenitor cells in the bone marrow which are critical for red blood cell development. Its expression occurs primarily in tissues with rapid cell turnover such as the bone marrow and B19 is also detectable in fetal liver tissues.
Biological function summary
Human parvovirus B19 acts by binding to the P antigen on susceptible cells particularly erythroid precursors. This interaction facilitates the virus's entry into the cell where it replicates and inhibits cellular DNA synthesis potentially causing cell apoptosis. The virus does not typically form part of a protein complex within the host cell but manipulates host cellular machinery to replicate its own DNA effectively. This mechanism highlights B19's influence in the development of certain anemic conditions such as transient aplastic crisis in patients with hemolytic disorders.
Pathways
The infection mechanism of human parvovirus B19 involves several cellular pathways including those associated with cell cycle regulation and apoptosis. Notably B19 impacts the retinoblastoma protein (pRb) related pathways which are important in controlling cell cycle progression. Furthermore the virus interferes with tumor suppressor protein p53 disrupting pathways related to DNA damage response. These interactions emphasize the virus's adeptness at manipulating host cell controls to ensure successful replication.
Infection with human parvovirus B19 has associations with erythema infectiosum and hydrops fetalis. In individuals with compromised immune systems or those with existing hemolytic anemia B19 infection can aggravate these conditions as the virus exacerbates the reduction of erythroid progenitor cells. The virus shares a relationship with the TNF-alpha protein particularly in inflammatory and autoimmune responses seen in parvovirus-associated arthralgia. This connection underlines the potential for B19 to contribute to a range of pathological conditions through direct and indirect interactions within the host.