Abcam, ab268695, Recombinant human KAT2B / PCAF protein (Active)

Size: 5µg
Recombinant human KAT2B / PCAF protein (Active) is a Human Fragment protein, in the 431 to 832 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268695 was 32 nmol/min/mg in a acetyltransferase assay using histone H3 peptide (1-21) as substrate.,
Accession:Q92831,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KAT2B also known as PCAF (p300/CBP-associated factor) is a histone acetyltransferase protein with a mass of approximately 94 kDa. It plays a role in chromatin remodeling by acetylating lysines on histone tails which facilitates transcriptional activation. KAT2B is expressed ubiquitously but higher expressions occur in tissues with active transcription such as the brain and testes. KAT2B/PCAF can also interact with transcription regulators adding another layer to its role in gene expression regulation.
Biological function summary
KAT2B/PCAF participates in modifying chromatin structure and impacting transcription. It forms part of various complexes most notably a complex with other proteins like p300 and CBP which have acetyltransferase activity too. Through modifying histones KAT2B/PCAF influences gene expression patterns critical for cell cycle regulation and differentiation. It also acetylates non-histone proteins playing roles in diverse biological processes beyond transcription.
Pathways
KAT2B/PCAF integrates into the regulation of cell cycle and apoptosis pathways. One of the major pathways it involves in is the p53 signaling pathway which is important for cell cycle arrest and apoptosis in response to DNA damage. KAT2B/PCAF works with proteins like p53 and HDACs (histone deacetylases) to fine-tune the balance between acetylation and deacetylation of histones and other proteins influencing cell cycle progression and cellular response to stress signals.
KAT2B/PCAF has associations with cancer and neurodegenerative diseases. Its capability to regulate cell cycle and apoptosis connects it to cancer biology where altered expression or activity might contribute to unchecked cell proliferation. Variations in KAT2B/PCAF activity also link to neurodegenerative disorders like Huntington’s disease. The protein interacts with other factors like p53 and SMAD transcription factors in contexts relevant to these diseases suggesting its roles in pathways critical for disease development or progression.