Abcam, ab268861, Recombinant human PI4K2B protein (Active)

Size: 5µg
Recombinant human PI4K2B protein (Active) is a Human Full Length protein, in the 1 to 481 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268861 was 1,573 nmol/min/mg in a kinase assay using PI:PS as substrate.,
Accession:Q8TCG2,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI4K2B also known as Phosphatidylinositol 4-kinase type 2 beta is an enzyme with a molecular mass of approximately 55 kDa. This protein plays a role in the phosphorylation of phosphatidylinositol converting it to phosphatidylinositol 4-phosphate (PI4P) an important step in the phosphatidylinositol signaling pathway. PI4K2B shows expression in a variety of human tissues with particularly high levels noted in the placenta and fibroblasts. Its activity influences the formation of different cellular membranes impacting various cellular processes.
Biological function summary
PI4K2B influences multiple cellular functions particularly those related to membrane trafficking and cell signaling. Although not part of a larger known complex PI4K2B's enzymatic production of PI4P has many downstream effects impacting endosomal dynamics and vesicular trafficking. The protein's involvement in membrane dynamics suggests important roles in regulating cell movement and recycling of membrane components affecting how cells respond to their surroundings.
Pathways
PI4K2B integrates into the broader phosphoinositide pathway that regulates cellular communication and processes. It closely interacts with proteins like AKT and RAB proteins which are essential in the regulation of vesicle transport and signal transduction. The production of PI4P by PI4K2B contributes to the modulation of the PI3K/AKT signaling pathway impacting cellular growth survival and metabolism. This pathway's complexity demonstrates PI4K2B's role in coordinating multiple biological reactions.
Studies link altered PI4K2B function to neurodegenerative diseases and cancer. Its aberrant activity influences the signaling pathways that contribute to the development and progression of these conditions. In the context of neurodegeneration PI4K2B interacts with proteins such as tau influencing the balance of endosomal transport in neurons. In cancer changes in its activity affect cellular proliferation and survival showing connections to other oncogenic proteins within signaling cascades. These implications highlight the importance of understanding PI4K2B's roles in health and disease.