Abcam, ab268866, Recombinant human PIK3C2G protein (Active)

Size: 5µg
Recombinant human PIK3C2G protein (Active) is a Human Fragment protein, in the 468 to 1203 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>80% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268866 was 5,000 nmol/min/mg in a kinase assay using PI:PS as substrate.,
Accession:O75747,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIK3C2G also known as phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing gamma polypeptide is an enzyme with a molecular mass of about 197 kDa. It falls under the class II PI3Ks characterized by their distinct regulatory and catalytic domain structures. PIK3C2G is expressed in various tissues including the liver heart and skeletal muscle. This protein plays a role in lipid signaling by phosphorylating phosphatidylinositols at the 3-position of their inositol ring which is important for multiple cellular processes.
Biological function summary
The protein PIK3C2G contributes to cell functions involving membrane trafficking and signal transduction. The protein performs its duties partly by participating in the formation of lipid signaling molecules. It does not generally form a stable complex with other proteins differentiating it from class I PI3Ks that typically form heterodimers. Instead PIK3C2G's activity strongly contributes to cellular tasks like the regulation of endocytosis and exocytosis processes critical for maintaining cellular homeostasis.
Pathways
PIK3C2G interacts within the phosphatidylinositol signaling pathway by regulating the synthesis of phosphorylated phosphatidylinositols. It serves a significant role alongside proteins like PIK3C2A and PIK3C2B in modulating intracellular signals. These activities relate closely to the AKT signaling pathway which controls cell growth and survival. PIK3C2G's position in these pathways is essential as it directly impacts how cells respond to external stimuli such as growth factors and hormones.
Altered expression or dysfunction of PIK3C2G can associate with metabolic and cardiovascular diseases. The enzyme has been connected to insulin resistance and complications arising from uncontrolled lipid metabolism. In cardiovascular disorders the proper regulation of PIK3C2G is vital for heart function as disruptions can lead to pathological heart conditions. The protein shares relevance with other proteins such as PIK3CA and PIK3CD which are similarly implicated in these metabolic and cardiovascular contexts.