Abcam, ab268869, Recombinant human PIP4K2 beta protein (Active)

Size: 5µg
Recombinant human PIP4K2 beta protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268869 was 5,250 nmol/min/mg in a kinase assay using PI(5):PS as substrate.,
Accession:P78356,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIP4K2 beta also referred to as phosphatidylinositol-5-phosphate 4-kinase type-2 beta is an enzyme that functions in the phosphorylation of phosphatidylinositol-5-phosphate (PI5P) to produce phosphatidylinositol-45-bisphosphate (PI(45)P2). The molecular mass of PIP4K2 beta is approximately 47 kDa. This kinase has important roles in cell signaling processes and maintains its activity across various tissues with expression noticeable in the brain liver and immune system cells.
Biological function summary
The generation of PI(45)P2 by PIP4K2 beta plays a critical role in membrane dynamics and actin cytoskeleton regulation. This enzyme contributes to the control of intracellular signaling by affecting pathways involved in cell growth survival and migration. PIP4K2 beta functions as part of larger protein complexes which can affect its activity and interactions coordinating cellular responses under different physiological conditions.
Pathways
PIP4K2 beta integrates into the phosphoinositide signaling pathway and influences the PI3K/AKT/mTOR signaling cascade imperative for cell survival and growth. It interacts with other kinases such as PI3K to regulate the availability of lipid signaling molecules. The activity of PIP4K2 beta is also linked to the regulation of insulin signaling working alongside phosphatases that modulate PI(45)P2 and its derivatives.
Dysregulation of PIP4K2 beta activity can associate with metabolic disorders like type 2 diabetes and is observed in certain cancer types. For instance abnormal expression levels can affect cellular proliferation and glucose metabolism highlighting its role in insulin signaling pathways. Additionally the interaction of PIP4K2 beta with proteins such as AKT an important player in cancer pathways highlights its potential impact on tumorigenesis and the development of insulin resistance.