Abcam, ab268871, Recombinant human PIP5K1 beta protein (Active)

Size: 5µg
Recombinant human PIP5K1 beta protein (Active) is a Human Full Length protein, in the 1 to 540 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268871 was 3,400 nmol/min/mg in a kinase assay using PI(4)P:PS as substrate.,
Accession:O14986,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The phosphatidylinositol-4-phosphate 5-kinase type 1 beta (PIP5K1β) also known as PIPK type I beta is an enzyme that plays an important role in the synthesis of phosphatidylinositol 45-bisphosphate (PIP2) from phosphatidylinositol 4-phosphate (PI4P). This enzyme has a molecular weight of approximately 68 kDa. It expresses across various tissues with significant presence in the brain and in hematopoietic cells. The PIP5K1β enzyme is important in regulating the levels of PIP2 a critical lipid in membrane dynamics and signaling.
Biological function summary
PIP5K1β acts within the phosphoinositide signaling pathway which is essential for a range of cellular processes including actin cytoskeleton organization vesicle trafficking and cell survival. It forms part of a complex with other kinases and phosphatases that modulate cellular lipid concentrations. PIP5K1β also contributes to signal transduction processes by participating in the recruitment and activation of proteins that respond to membrane-associated signals.
Pathways
PIP5K1β is heavily involved in the regulation of the phosphoinositide 3-kinase (PI3K) pathway and the actin cytoskeleton pathway. Its product PIP2 serves as a substrate for PI3K which generates phosphatidylinositol-345-trisphosphate (PIP3) an important signaling molecule. PIP5K1β therefore indirectly impacts pathways that involve proteins like AKT and Rac1 which are important to cellular growth and motility.
PIP5K1β connects to cancer and immune system-related disorders. Dysregulation of PIP5K1β activity can lead to aberrant PI3K signaling contributing to oncogenic processes. Additionally it affects proteins such as AKT which plays a role in tumor progression and survival. In immune disorders altered PIP5K1β levels can impact the actin cytoskeleton affecting immune cell dynamics and function.