Abcam, ab268890, Recombinant human PRMT6 protein (Active)

Size: 5µg
Recombinant human PRMT6 protein (Active) is a Human Full Length protein, in the 1 to 375 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268890 was 1.4 nmol/min/mg in a methyltransferase assay using histone H3 peptide (1-21) as substrate.,
Accession:Q96LA8,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRMT6 or Protein Arginine Methyltransferase 6 is an enzyme that adds methyl groups to arginine residues on target proteins. It is a member of the PRMT family and has a molecular mass of approximately 42 kDa. This enzyme is widely expressed in nucleous where it modifies histones and various nuclear proteins influencing chromatin structure and gene expression.
Biological function summary
PRMT6 plays a pivotal role in regulating transcription by methylating histone H3 at arginine 2 (H3R2) which suppresses gene expression. It forms part of larger chromatin remodeling complexes that modulate access to DNA. PRMT6 also interacts with p53 an important regulator of the cell cycle impacting its transcriptional activity and stability.
Pathways
PRMT6 is central to epigenetic and transcriptional regulation pathways. It functions alongside proteins like p53 and Mdm2 within the DNA damage response pathway where it influences cell proliferation and repair processes. This enzyme further relates to the Akt signaling pathway which affects cell growth and survival although it is not the primary pathway.
PRMT6 is implicated in cancer and systemic lupus erythematosus (SLE). In cancer deregulation of PRMT6 can lead to uncontrolled cell growth by affecting genes related to cell cycle and proliferation often interacting with protein p53. In systemic lupus erythematosus altered PRMT6 activity may contribute to abnormal immune responses associating with proteins involved in autoimmunity.