Abcam, ab268960, Recombinant human SETD6 protein (Active)

Size: 5µg
Recombinant human SETD6 protein (Active) is a Human Full Length protein, in the 1 to 473 aa range, expressed in Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>80% SDS-PAGE,
Expression system:Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268960 was 380 pmol/min/mg in a methyltransferase assay using histone H2A protein as substrate.,
Accession:Q8TBK2,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SETD6 also known as SET Domain Containing 6 is a protein methyltransferase involved in the regulation of protein activities through methylation. The protein has a molecular mass of approximately 46 kDa. SETD6 predominantly localizes in the nucleus but can also be found in other cellular compartments depending on cellular conditions. The expression is relatively ubiquitous present in various tissues and cell types indicating its diverse functions in different biological contexts.
Biological function summary
The protein functions as a monomer and it methylates the lysine residues of target proteins which influences their interactions and functionality. Its enzymatic activity plays a critical role in chromatin modification affecting transcriptional regulation by altering the chromatin state. SETD6 modifies several proteins including the nuclear factor NF-κB subunit RELA which impacts inflammatory responses and cell survival. Through its methylation activity SETD6 modulates important cellular processes by changing the structure and function of the chromatin.
Pathways
Scientists place SETD6 in significant cellular pathways such as the NF-κB signaling pathway and chromatin remodeling pathway. Within the NF-κB pathway SETD6 methylation results in repression of NF-κB target genes controlling inflammatory responses. In chromatin remodeling it interacts with proteins such as WDR5 a critical component of the MLL/COMPASS complex to regulate transcription and maintain chromatin structure. These interactions connect SETD6 to wider cellular functions that influence cell fate and response to environmental stressors.
Researchers identify SETD6 dysregulation as a factor in cancer and inflammatory diseases. In cancer abnormal SETD6 activity can lead to altered expression of oncogenes and tumor suppressor genes contributing to tumorigenesis. Connections exist between SETD6 and other cancer-related proteins such as β-catenin influencing pathways linked to cell proliferation and cancer progression. In inflammatory disorders SETD6 modifies RELA impacting the NF-κB pathway and potentially exacerbating inflammatory responses. Understanding SETD6 interactions in these contexts could lead to therapeutic strategies targeting its methylation activity.