Abcam, ab269114, Recombinant human USP11 protein (Tagged)

Size: 5µg
Recombinant human USP11 protein (Tagged) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab269114 protein is 136 kDa.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity of ab269114 was determined to be 47 nmol/min/mg in a DUB assay using recombinant human ubiquitin-based proluciferin substrate.,
Accession:P51784,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
Ensure the validity of your result using our bioactive recombinant human USP11 protein (GST-Tagged) ab269114 as a control in SDS-PAGE.
Check out our protein gel staining guide for SDS-PAGE

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP11 also known as ubiquitin specific peptidase 11 is a deubiquitinating enzyme with a molecular mass of approximately 110 kDa. It removes ubiquitin molecules from target proteins regulating their stability and function. This enzyme is part of the USP family and exhibits strong expression in tissues like the testes brain and thyroid. The deubiquitination process is significant in maintaining protein homeostasis by recycling ubiquitins and controlling protein degradation.
Biological function summary
Ubiquitin-specific peptidases such as USP11 participate in diverse cellular functions including DNA repair signal transduction and cell cycle progression. USP11 often operates as part of a larger regulatory complex. It modulates DNA repair by interacting with proteins involved in the repair processes such as BRCA2. Through its deubiquitination activity USP11 significantly influences cellular response to DNA damage and helps to maintain genomic stability.
Pathways
USP11 plays a role in key mechanisms like the DNA damage response and NF-kB signaling pathways. It interacts with proteins involved in these pathways offering a layer of regulation. In the DNA damage response pathway USP11 associates with BRCA2 assisting in the repair of double-stranded breaks. In the NF-kB signaling pathway its activity can affect the degradation of IkB which allows the transcription factor NF-kB to enter the nucleus and promote gene expression.
Aberrations in USP11 function have been linked to cancer and neurodegenerative diseases. In cancer alterations in USP11 might contribute to tumorigenesis by affecting the DNA repair pathways especially through the BRCA2 protein interactions. Neurodegenerative diseases may also be connected to USP11 dysfunction due to its role in the degradation of proteins that accumulate in these conditions. The enzyme's interaction with various signaling proteins suggests a potential target for therapeutic interventions in these diseases.